Influence of chlorine dioxide on cell death and cell cycle of human gingival fibroblasts.
نویسندگان
چکیده
OBJECTIVES The effects of chlorine dioxide (ClO2), sodium hypochlorite (NaOCl), and hydrogen peroxide (H2O2) on cell death and the cell cycle of human gingival fibroblast (HGF) cells were examined. METHODS The inhibition of HGF cell growth was evaluated using a Cell Counting Kit-8. The cell cycle was assessed with propidium iodide-stained cells (distribution of cells in G0/G1, S, and G2/M phases) using flow cytometry. The patterns of cell death (necrosis and apoptosis) were analyzed using flow cytometry with annexin V-FITC/PI staining. RESULTS The lethal doses for 50% of the cells (LD50) of ClO2, NaOCl, and H2O2 were 0.16, 0.79, and 0.11 mM, respectively. All three dental disinfectants induced G0/G1 cell cycle arrest. H2O2 induced apoptosis at concentrations of 0.05 and 0.1 mM, while NaOCl and ClO2 did not induce significant apoptosis at any concentration examined. CONCLUSIONS These results suggest that ClO2 is sufficient for use as a dental disinfectant compared with H2O2 or NaOCl.
منابع مشابه
The Effects of Low Level Laser Therapy on the Expression of Collagen Type I Gene and Proliferation of Human Gingival Fibroblasts (Hgf3-Pi 53): in vitro Study
Background Recent investigations show that both proliferation and secretion of macromolecules by cells can be regulated by low level laser therapy (LLLT). The aim of this study was to determine whether LLLT could induce a bio-stimulatory effects on human gingival fibroblasts (HGF3-PI 53). Therefore, the effect of laser irradiation on human gingival cell proliferation and collagen type I gene ...
متن کاملThe Effects of Low Level Laser Therapy on the Expression of Collagen Type I Gene and Proliferation of Human Gingival Fibroblasts (Hgf3-Pi 53): in vitro Study
Objective(s): Recent investigations show that both proliferation and secretion of macromolecules by cells can be regulated by low level laser therapy (LLLT). The aim of this study was to determine whether LLLT could induce a bio-stimulatory effects on human gingival fibroblasts (HGF3-PI 53). Therefore, the effect of laser irradiation on human gingival cell proliferation and collage...
متن کاملComparison of the Cytotoxic Effects of Nanoparticulate and Microparticulate Calcium Sodium Phosphosilicate Mouthwashes on Human Gingival Fibroblasts: an in-vitro Study
Introduction: This study sought to assess the cytotoxic effects of nanoparticulate and microparticulate calcium sodium phosphosilicate mouthwashes on human gingival fibroblasts (HGFs). Methods: This in vitro study was conducted on HGFs isolated and cultured in a 48-well plate containing standard culture medium for evaluation of four concentrations of the two m...
متن کاملبررسی اثر سمیت سلولی عامل باندینگ عاجی AdheSE
Background and Aim: An important requirement for a dentin bonding agent is biological compatibility. Since dentin bonding agents are placed in cavity preparations with subgingival extensions, with direct contact to gingival and mucosal tissues, tissue response to these materials must be investigated. The aim of this study was to examine the cytotoxicity of AdheSE, a self etching adhesive, on hu...
متن کاملاثرات گاز کلر بر سیکل سلولی و محتوی DNA گلبولهای سفید موش سوری The Effects of Chlorine on Cell Cycle and DNA Content of WBC in Mice
Background & Aim: Chlorine has been known as a mucus membranes and respiratory tract irritant. This gas can increase free radicals which cause cell damage. The aim of the present study was to measure DNA content and cell cycle in white blood cells after chronic chlorine poisoning. Materials & Methods: A clinical experimental study was carried out on 80 male mice(40 mice as sample and 40 as ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of dentistry
دوره 36 12 شماره
صفحات -
تاریخ انتشار 2008